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Ernst Dieter Wachsmuth Jean-Pierre Jost 《Biochimica et Biophysica Acta (BBA)/General Subjects》1976,437(2):454-461
The localization of albumin and vitellogenin was determined in liver sections from control and estradiol-treated chickens by two different immunocytochemical techniques: (1) The sandwich technique with rabbit anti-lipovitellin or rabbit anti-albumin IgG and fluorescent goat anti-rabbit IgG and (2) the mixed aggregation immunocytochemical technique with anti-lipovitellin IgG and fluorescent lipovitellin.The results show that the antibody against albumin bound only to all liver parenchymal cells. Furthermore, the fluorescence intensity was equally strong in the portal, intermediate and central zones of the lobules.The fluorescent stain for vittelogenin was not above background in livers of control chicks but was far above background in estradiol-treated chicks. As with albumin the fluorescent stain was distributed equally among the parenchymal cells.The results were quantitatively the same 2 and 4 days after estradiol treatment. The relative rates of synthesis and the concentrations of albumin and vitellogenin correlate well with values obtained for tissue sections by immunocytochemical techniques. 相似文献
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Abstract Investigations on the circadian cycle of secretion in the neurosecretory nuclei of mouse. The circadian cycle of secretion of both hypothalamic neurosecretory nuclei N. supraopticus and N. paraventricularis in mice was studied by histological, histochemical, biochemical and electron-microscopical methods. Both areas undergo a circadian secretion cycle (24 h-rhytm). The activity of metabolism increases at the beginning of night, whereas by day the cells are considered to be in a phase of restitution and transportation of the secretion. Especially increases the volume of the cell nuclei (synthesis of RNA and proteins) and the activity of the extralysosomal unspecific esterase rises to maximal values. From midnight till the beginning of the light-phase the cells show a maximal volume of the nuclei and an increased secretion as well as higher activity of acid phosphatase and lactate dehydrogenase. Unspecific esterase activity, on the other hand, diminished. There was a noticable increase in the quantity of neurosecretion in both areas, it reached a maximum at the beginning of the light-phase, whereas the volume of the nuclei and the activity of acid phosphatase and thiamine pyrophosphatase diminished. The neurosecretion is more and more transported to the hypophysis. The cells restitute during the light-phase and reach their starting level. 相似文献
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